Cells exposed to TTFields exhibit an inability to progress through anaphase successfully resulting in cellular derangement.

<p>MDA-MB-231 cells were synchronized using double aphidicolin block and then allowed to re-enter the cell cycle in the absence of TTFields (top panels), exposed to TTFields (middle panels) or treated with 3 μM paclitaxel (bottom panels). Cells were harvested and analyzed by FACS for transit through mitosis by staining with antibodies against Cyclin B antibodies and pH3 at 4, 7, 9, 11 and 13 hours after aphidicolin removal. Cells treated with TTFields did not exhibit a marked accumulation of mitotic cells showing a slight increased accumulation of cells at the 9 and 11 hour time points compared to control cultures suggesting a reduction in the rate of mitotic exit (<b>A</b>). Results are representative of 4 separate experiments. MDA-MB-231 cells were synchronized using aphidicolin followed by RO3306 treatment and were then allowed to progress through mitosis n the presence or absence of TTFields following drug removal. Cells were then collected at 60, 120 and 150 min and stained with antibodies against pH3 and 7-AAD for DNA content for analysis by FACS (<b>B</b>).