Effect of CD47 on inflammatory gene expression induced by candidemia.

<p>mRNA abundance was determined by qPCR using cDNA synthesized from total RNA of spleens and kidneys. Uninfected WT and <i>cd47</i>^-/- mice were compared with the infected WT and <i>cd47</i>^-/- mice. Each group had at least three mice. Cxcl2, IL-10 and Fos expression in spleens were significantly up-regulated in infected CD47 mice compared with the WT infected mice (p> 0.001, .01 and 0.01 respectively). CCR2 and TRAF 2 of infected <i>cd47</i>^-/- kidney tissues were significantly down-regulated compared with the infected WT mice, while IL23R was significantly up regulated in infected <i>cd47</i>^-/- mice (p> 0.001, 0.05 and 0.01 respectively). Results from three mice per group were analyzed using two-way ANOVA with post Bonferroni comparison test.