The PCM scaffolding proteins Spd-2 and Cnn are required for efficient aMTOC formation.

<p>(A) <i>Sas-4</i> mutant cells and <i>Spd-2 Sas-4</i> and <i>cnn; Sas-4</i> double mutant cells were stained with antibodies against Cnn (red) and γ-tubulin (green). DNA is in blue. Scale bar represents 5μm. The aMTOCs present in <i>Sas-4 and Spd-2 Sas-4</i> mutant cells are indicated by arrows. (B) Quantification of aMTOC formation in mitotic brain cells (as judged by γ-tubulin staining on at least one spindle pole) shows that γ-tubulin staining on spindle poles can be detected in 50.69% ± 0.83 of <i>Sas-4</i> mutant cells, 27% ± 4.33 of <i>Spd-2 Sas-4</i> double mutant cells and 1.23% ± 0.67 of <i>cnn; Sas-4</i> double mutant cells (a minimum of 80 cells from 3 brains were quantified per genotype). (C) Quantification of Cnn recruitment to mitotic spindle poles shows that Cnn staining on spindle poles can be detected in 50.69% ± 0.83 of <i>Sas-4</i> mutant cells and 27% ± 4.33 of <i>Spd-2 Sas-4</i> double mutant cells. (A minimum of 137 cells from 4 brains were quantified per genotype). All data are expressed as mean±SEM with average per brain representing one data point. P-values were calculated using a Mann-Whitney test.