Mitochondrial reshaping accompanies neuronal differentiation in the chicken neural tube.

<p>Panels A through E represent confocal micrographs of green OXPHOS-stained mitochondria in E3.5 transversal sections (scale bar 50 μm), magnified in the mid- and right-side panels (scale bar 10 μm). In panels A, C, and E, SR101-Phalloidin signaling (red) was used to mark the cellular contours. Neuronal progenitors, immunolabeled either with Olig2 for future motor neurons (A, blue) or Pax6 for future interneurons (C, blue), contain short and thick mitochondria. In panel B, JC7-immunodetection (purple) delineates the surface of differentiating motor neurons endowed with a longer and denser mitochondrial network. In panel D, the mitochondrial network of beta3-tubulin-stained differentiating neurons (purple) is also long and dense. In panel E, P-H3-immunostained (blue) mitotic progenitors are shown to contain very small and round mitochondria.