Chromatin structure observed in the SEVENS assay.

<p>(A-C) The SEVENS assays revealed distinct chromatin structures in the <i>CYP19</i> locus of HepG2 (A), KGN (B), and HeLa cells (C). The proportion of open and closed chromatin is represented as an enrichment ratio of a given sequence in upper fractions to that in lower fractions (Up/Lo) using a log2 scale (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0128282#sec002" target="_blank">Materials and Methods</a>). The gene structure of the <i>CYP19</i> locus is drawn above each chart. Each vertical line extends from a promoter region to note a corresponding position in the respective lower charts. Red circles and blue rectangles denote the Up/Lo values of active and repressed reference loci, respectively, which are abbreviated as TU, AC, GA, OR, MY, or IL for the <i>TUBB</i>, <i>ACTB</i>, <i>GAPDH</i>, <i>OR1A1</i>, <i>MYT1</i>, or <i>IL2RA</i> loci, respectively. To compare assessed regions of the <i>CYP19</i> locus to these references, red and blue belts are placed on the charts to indicate the ranges of the Up/Lo values of the references. The positions referred to in the text are labeled as “<u>e</u>nrichment in <u>u</u>pper fractions of the <u>S</u>EVENS assay (EUS)” in the charts.