Expression analyses of viral structural proteins in infected cells.

<p>(A) Sf9 cells were infected with vAcΔ<i>cc</i> or vAcΔ<i>cc</i>Δ<i>fp25k</i> at an MOI of 5. Infected cells were collected at 48 h p.i. and analyzed by western blotting using the corresponding antibodies, the results were repeated twice. (B) Sf9 cells infected with vAcΔ<i>cc</i> or vAcΔ<i>cc</i>Δ<i>fp25k</i> (MOI = 5) were collected at 48 h p.i. and analyzed by qRT-PCR. The transcriptional levels of viral genes were normalized to the internal control <i>28S</i> rRNA, and the transcription difference between vAcΔ<i>cc</i>- and vAcΔ<i>cc</i>Δ<i>fp25k</i>-infected cells was analyzed by the 2^{<i>-ΔΔC</i>}T method. The results of corresponding genes in vAcΔ<i>cc</i>-infected cells were set as 100%. The data are from three independent experiments. Error bars represent standard deviation. Data were analyzed by two-tailed Student's <i>t</i>-test. <b>*</b><i>P</i><0.05.