Ly6C^- monocytes through IL-10 production limit TNF production by Ly6C⁺ monocytes in <i>T</i>. <i>congolense</i>-infected mice.

<p><b>(A)</b> Liver Ly6C⁺ monocytes (Ly6C⁺ Mo) and MHC-II^{- to lo} Ly6C^- monocytes (Ly6C^- Mo) purified from CX₃CR1-GFP^+/- mice at day 7 pi and 21 pi, respectively, were cultured alone or together at indicated ratio in presence of control (-) or neutralising anti-IL-10R antibody. TNF concentration in cell supernatants was measured. Data are shown as mean + SD of 1 out of five independent experiments. * p<0.05 compared to Ly6C⁺ monocytes cultured alone, § p<0.05 comparing populations linked by horizontal bar. <b>(B-D)</b> Liver MHC-II^{- to lo} Ly6C^- monocytes gated as described in Fig 5A in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004873#ppat.1004873.s001" target="_blank">S1 Text</a> purified from IL-10^wt/wt LysM-cre^+/+ or IL-10^flox/flox LysM-cre^+/+ mice at day 21 pi were transferred in CD45.1 WT mice at day 4 and day 6 pi. Control mice received HBSS. Different parameters were evaluated in recipient mice at day 7 pi: <b>(B)</b> TNF levels in liver Ly6C⁺ monocytes determined as mean fluorescence intensity difference (dMFI) between anti-TNF and isotype control antibodies, <b>(C)</b> Spontaneous TNF concentration in liver non-parenchymal cell culture supernatants, and <b>(D)</b> ALT levels in blood serum. Data are shown as mean + SD of 3 individual mice of one representative out of three independent experiments. § p<0.05 comparing populations linked by horizontal bar.