Depletion of Ly6C^- monocytes in Nr4a1^-/- mice increases TNF production and liver damage in <i>T</i>. <i>congolense</i>—infected mice.

<p>Different parameters were evaluated in WT and Nr4a1^-/- mice at day 21 pi. Liver Ly6C⁺ monocytes (Ly6C⁺ Mo), Ly6C^- monocytes (Ly6C^- Mo) and macrophages (MF) were gated as in S5A Fig in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004873#ppat.1004873.s001" target="_blank">S1 Text</a>. <b>(A)</b> Numbers of Ly6C⁺ monocytes, Ly6C^- monocytes and macrophages within liver non-parenchymal cell population. <b>(B)</b> Spontaneous TNF levels in Ly6C⁺ monocytes and CD11b⁺ Ly6C^- cells determined as mean fluorescence intensity difference (dMFI) between anti-TNF and isotype control antibodies. <b>(C,D)</b> TNF and <b>(F,G)</b> IL-10 concentration in <b>(C,F)</b> supernatants of liver non-parenchymal cell cultures or <b>(D,G)</b> in blood serum. <b>(E)</b> ALT levels in blood serum. Data are shown as mean + SD of 3 individual mice of one representative out of five independent experiments. *p<0.05 compared to WT mice.