Nanotrap particles can protect NP from degradation at increased temperatures and times.

<p>A and B) Cytoplasmic extracts from RVFV infected cells were diluted in 100% sheep serum for a final concentration of 7.7 μg/ml. C and D) Viral supernatant was diluted in 100% goat serum for a final titer concentration of 1E+06 pfu/ml. One milliliter of sample was incubated with NT45 for 24 to 120 hours at either ambient temperature (A and C) or at 37^°C (B and D). No Nanotrap particle (-NT) and control cytoplasmic extract (CE) samples at 7.7μg/ml or 0.77 μg/ml (B only) at 10 μl volumes were processed in parallel. Viral supernatants without Nanotrap particles containing samples were analyzed on a separate gel (E). After the incubation time, the (+)NT samples were centrifuged and washed once with 0.25M sodium thiocyanate and twice with diH₂O. Presence of NP was analyzed by western blot using antibodies against NP.