Sequence comparison of putative antimicrobial peptides from <i>U</i>. <i>yaschenkoi</i> and <i>U</i>. <i>manicatus</i>.

<p>Multiple alignment of sequences obtained from the transcriptome of <i>U</i>. <i>yaschenkoi</i> that codify antimicrobial peptides from the subfamily NDBP-5. These sequences are compared with CYLIP-Uro-1 (GenBank: GALI01000003.1), CYLIP-Uro-2 (GenBank: GALI01000004.1), CYLIP-Uro-3 (GenBank: GALI01000005.1), CYLIP-Uro-4 (GenBank: GALI01000006.1) and CYLIP-Uro-5 (GenBank: GALI01000007.1) from <i>U</i>. <i>manicatus</i> [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0127883#pone.0127883.ref049" target="_blank">49</a>]. The predicted signal peptide is underlined; the mature peptide is in bold and highlighted in yellow, the conserved proteolytic site GKR is in italics and underlined and the propetide in italics. The hyphen (-) in the name of <i>U</i>. <i>yaschenkoi</i> sequences indicates that the amino acid sequence was found within different nucleotide sequences (transcripts), for example: comp17_c0_seq1-4 means that four different nucleotide sequences codify the same peptide. The percentage of identity of the mature peptide is indicated at the right (% Identity) with respect to the peptide encoded by comp17_c0_seq1-4; additionally, at the far right, the theoretical molecular weights of the antimicrobial peptides for <i>U</i>. <i>yaschenkoi</i> are shown. The theoretical molecular weight of the peptide encoded by the sequences comp17_c0_seq1-4 (in bold) has a perfect match with the <i>U</i>. <i>yaschenkoi</i> proteome previously reported by [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0127883#pone.0127883.ref041" target="_blank">41</a>] at the retention time 13.88. Note: several sequences of transcripts found in the <i>U</i>. <i>yaschenkoi</i> transcriptome codify the same precursors, such as: comp17_c0_seq1-4 and comp18_c0_seq1-2 (not shown); comp17_c0_seq5 and comp18_c0_seq3-4 (not shown); comp192_c0_seq1-2, comp192_c0_seq4-5 (not shown), comp192_c0_seq7-9 (not shown) and comp196_c0_seq1-7 (not shown) codify the same precursor.