Frequency of LMP1- or LMP2a-specific immune responses by ELISA and comparison of IFN-γ ELISPOT assay.

<p>(A) Frequencies of IFN-r (n = 20), TNF-a (n = 22) and IL-2 (n = 20) levels were investigated with supernatant which were secreted in CD8⁺ and CD4⁺ T cells stimulated with LMP1 or LMP2a mRNA-transfected DCs. Box and whisker plots (Tukey’s test; 25% ~ 75%) indicate median (middle line in the box) of secreted cytokine level in individuals. (B) Comparison of estimates of cellular immune responses obtained using ELISPOT and cytokine ELISA. The results of the IFN-γ ELISPOT assays correlated well with those from IFN-γ ELISA (n = 20, r = 0.6595, Pc < 0.0001). (*: p < 0.05, **: p < 0.01, ***: p < 0.0001)