Effects of anti-RAGE and MAPKs inhibitor on AGEs-induced down-regulation of PPARγ in primary human chondrocytes.

<p>In A, chondrocytes were pretreated with anti-RAGE (5 μg/ml) for 12 hours before AGEs (100 μg/ml) stimulation. In B, chondrocytes were pretreated with SB203580, SP600125, PD98059 (10 μM) for 30 minutes prior to AGEs (100 μg/ml) stimulation. The expression of PPARγ was quantified by real-time PCR and western blotting using β-actin as an internal control. In A, densitometric analysis for PPARγ levels corrected to β-actin is shown. All data are expressed as means ± SD and are representative of three independent experiments. *: <i>p< 0</i>.<i>05</i> versus control, #: <i>p< 0</i>.<i>05</i> versus AGEs treatment, &: <i>p> 0</i>.<i>05</i> versus AGEs treatment.