HFD led to impairment of IRS-1/Akt pathway and activation of inflammatory responses in primary hepatocytes.

<p>Primary hepatocytes were isolated from mice fed a normal diet or HFD for 12 weeks (as described in the Materials and Methods section). (A) Representative western blots of IRS-1/Akt pathway proteins and the respective densitometry measurements; (B) Representative western blots of inflammatory proteins and the corresponding densitometry measurements. Data presented as mean ± SD, <i>n</i> ≥3 mice/group, *<i>p</i> < 0.05, ** <i>p</i> < 0.01 <i>versus</i> control group.