Effect of Paclitaxel upon the differentiation ability of hMSCs.

<p>(A) and (B) After 21 days of induced adipogenic differentiation in the presence of either 0, 10, or 10,000 nM Paclitaxel, cells were fixed and stained with the lipophilic dye Nile Red and Hoechst 33342. Image samples were taken of each culture and area of staining was standardized to nuclei counts for that area. A decrease in lipid accumulation of approximately 40% when compared to control was observed in hMSCs treated with either 10 or 10,000 nM Paclitaxel. (C) Microtubule staining via immunocytochemistry reveled an apparent dose-dependence upon cytoskeletal organization in the differentiated hMSCs. At 10 nM characteristic bundling was observed. At 10,000 nM over-accumulation of microtubules was observed. Results are displayed as mean ± standard error. *** P < 0.01. Scale bar (B) 150 μm, (C) 15 μm.