Characterization of the fibroblast-like state hMSCs adopt when treated with Paclitaxel.

<p>(A) After 72hrs of exposure to either 10 or 10,000 nM Paclitaxel hMSCs transition from the typical spindle-shaped morphology, seen in the untreated hMSCs, to one that is more broad and flat. Quantitative Real-Time PCR was used to determine expression levels of (B) CD9 (C) CD106 (D) CD146 (E) CD166 (F) integrin alpha 11 (ITGA11) (G) matrix metalloproteinase 1 (MMP-1) and (H) MMP-3 in hMSCs treated with 10 nM Paclitaxel over time. Results are displayed as mean ± standard deviation. ** P < 0.05, *** P < 0.01. Scale bar (A) 150 μm.