Registration based image matching of μCT and histological section stained with Movat’s Pentachrome.

<p>A. shows the histological section imaged using a 10x objective on a Dot-Slide scanning system. The initial image required deformation, rotation and translation using UnwarpJ elastic registration [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0126230#pone.0126230.ref023" target="_blank">23</a>] to match the μCT image, reflecting deformation during sectioning. The final transformed image of the histological section (B) and with a matching slice from the corresponding μCT image (C). Details of the same sub-region in A, B and C are shown in D, E and F. Structural details in the μCT image correspond closely to those seen by light microscopy.