Assessment of the interaction of Ang-(1–7) and ROS in cultured pancreatic rudiments.

<p>(A-B) DHE-labelled sections. (C-D) Assessment of mRNA expression changes of NADPH oxidase subunits <i>Nox1</i>, <i>Nox2</i> and <i>Nox4</i>. (E-F) IHC for Nox4. (G) Protein expression of p22^phox in pancreatic rudiments treated with 1μM A779 (Mas receptor antagonist) in the presence and absence of 1 μM Ang-(1–7). (H) mRNA expression of <i>Ngn3</i> and <i>Insulin</i> in pancreatic rudiments treated with 1 μM Ang-(1–7) in the presence and absence of 0.5 μM DPI (Nox4 inhibitor). Scale bar = 50 μm. One-way ANOVA followed by Tukey’s <i>post hoc</i> tests were used. All data are expressed as means ± SEM. n = 5 per group. *<i>p</i> < 0.05, **<i>p</i> < 0.01, *** <i>p</i> < 0.001 <i>vs</i>. control, ^#<i>p</i> < 0.05, ^##<i>p</i> <0.01 <i>vs</i>. Ang-(1–7).