ND1 labels specific subset of neurons in the mitral and glomerular layers.

<p><b>A-</b> Immunohistochemistry of sagittal OB section of 2-week old ND1^+/LacZ mice shows β-Gal⁺ cells do not co-express CalR, CalB, TH, Pax6, Meis and SP8 which are markers associated with GABAergic glomerular and periglomerular identities. Lower panels represent higher magnification images of framed areas. <b>B-</b> Rare β-Gal⁺/ParV⁺ neurons are identified in the external plexiform layer at P15. <b>C-</b> At 2 weeks of age, approximately a third of all Tbr2⁺ cells stain for β-Gal in the GL (27% ± 2.3%; 1942 cells counted in total) and MCL (20.6% ± 2.3%; 735 cells counted in total). Conversely, nearly all β-Gal⁺ cells co-express Tbr2 in the GL (98.3% ± 0.8%) and the MCL (99.3% ± 0.7%). Immunohistochemistry for ND1 on wildtype tissue confirms ND1 is specifically expressed in specific subset of Tbr2⁺ neurons in the mitral cell layer and glomeruli. <b>D-</b> β-Gal⁺ neurons are immuno-positive for the glutamatergic markers VGluT2. <b>E-</b> Tbr1⁺ neurons define subsets of OB glutamatergic neurons, which do not originate from a ND1 lineage. <b>F-</b> Permanent lineage tracing confirms the existence of both ND1-specific subtype diversity among Tbr2⁺ neurons and ParV⁺ neurons originating from ND1-positivelineage. <b>G-</b> Model summarizing origin, migration and location of ND1 progenitors and progenies at embryonic day E15.5 and postnatal stage. <b>A- to F-</b> Lower panels represent higher magnification images. SVZ = subventricular zone, MCL = mitral cell layer, RMS = rostral migratory stream, GCL = granule cell layer, GL = glomeruli, EPL = external plexiform layer, V = ventricle, OB = olfactory bulb. Values shown as mean ± s.e.m., <i>n</i> = 3 animals. Scale bars: 50 μm (A-F).