Shows the phenotypic analysis of cultured spleen- and bone marrow-derived macrophages.

<p>Splenocytes and bone marrow cells were isolated from GFP^<b>+</b> mice. The cells were seed into culture dishes with splenocytes seeding density 6 times higher than bone marrow cells. The cells were cultured in RPMI/10%serum containing M-CSF. After reaching confluencey, cells were placed for 24 hrs. in the media free of serum or M-CSF. The cells were treated with indicated reagents followed by RNA extraction and analysis. This figure shows the expression of transcription factors known to affect macrophage polarization in the two macrophage sources.