Real-time PCR is applied and relative gene expression levels are determined by the comparative Ct method.

<p>Bars indicate the means±S.E. of ratios of the relative expressions of the target mRNA to that of GAPDH. The mean of the control is arbitrarily set at 1. Statistical significance is indicated by * (p<0.05) or ** (p<0.01). A. LNCap cells were transduced with control-siRNA (C) or Pin1 siRNA (P) for 48hr. B. DU145 cells were transduced with control-siRNA (C) or Pin1 siRNA (P) for 48hr.