Enhanced differentiation of cancer stem-like cells (CSCs) of glioblastoma multiforme by arsenic trioxide and 10058F4.

<p>Immunofluorescent analysis for Nestin (green), Olig2 (red), TujI (green), GFAP(red) and DAPI staining of nuclei (blue) (A) and quantitative analysis of Nestin-positive, Olig2-positive, Tuj1-positive, GFAP-positive cells (B) in cancer stem-like cells (CSCs) of glioblastoma multiforme (GBM) (RI01) 3days after treatment with or without 2μM arsenic trioxide or 60μM 10058F4 in the presence of 1% FCS. Scale bar = 100μm. The data is the mean ± S.D. *P = 0.021, **P = 0.012, ***P = 0.00055, ^##P = 0.024, ^###P = 0.0036, ^§P = 0.031, ^§§P = 0.050.