<p>Five cases with matched tumor—blood samples are shown. Annotations in black letters are given for tumors (FFPE); in blue letters for germline (blood). TCC: approximate tumor cell content in the DNA sample; VC and PC: variant and position coverage with MPS; VAF: Variant frequency (VC/PC). The same DNA samples were used per case for both methods. A—C: same case, DNA quality unfavourable, three variants in tumor, two in germline. D—F: individual cases with unfavourable (D and E) and favourable (F and G) DNA quality. Sanger sequencing peaks usually but not always corresponded to MPS VAF. In A, D and F, perhaps in B as well, variants would have been missed with Sanger sequencing only. Note target specific differences in position coverage, whi...
<p>FN: false negative result, extra: additional low allele frequency variants identified compared to...
The accurate detection of low-allelic variants is still challenging, particularly for the identifica...
Next-generation sequencing (NGS) has enabled the high-throughput discovery of germline and somatic m...
<p>Of the filtered 41 candidates, 21 variants were confirmed by Sanger sequencing. Two examples are ...
Quantification of tumor‐specific variants (TSVs) in cell‐free DNA is rapidly evolving as a prognosti...
Use of next-generation sequencing to detect somatic variants in DNA extracted from formalin-fixed, p...
Quantification of tumor-specific variants (TSVs) in cell-free DNA is rapidly evolving as a prognosti...
Quantification of tumor-specific variants (TSVs) in cell-free DNA is rapidly evolving as a prognosti...
Quantification of tumor-specific variants (TSVs) in cell-free DNA is rapidly evolving as a prognosti...
Quantification of tumor-specific variants (TSVs) in cell-free DNA is rapidly evolving as a prognosti...
Quantification of tumor-specific variants (TSVs) in cell-free DNA is rapidly evolving as a prognosti...
Next-generation sequencing (NGS) has rapidly replaced Sanger sequencing as the method of choice for ...
<p>Sanger sequence validation of highly expressed novel somatic SNVs for <i>MRPL3</i> variant in the...
<p>(A) Mapped reads of a Patient with RCMD loaded in the IGV browser (Version 2.3.34). <i>TET2</i> p...
Next-generation sequencing (NGS) has enabled the high-throughput discovery of germline and somatic m...
<p>FN: false negative result, extra: additional low allele frequency variants identified compared to...
The accurate detection of low-allelic variants is still challenging, particularly for the identifica...
Next-generation sequencing (NGS) has enabled the high-throughput discovery of germline and somatic m...
<p>Of the filtered 41 candidates, 21 variants were confirmed by Sanger sequencing. Two examples are ...
Quantification of tumor‐specific variants (TSVs) in cell‐free DNA is rapidly evolving as a prognosti...
Use of next-generation sequencing to detect somatic variants in DNA extracted from formalin-fixed, p...
Quantification of tumor-specific variants (TSVs) in cell-free DNA is rapidly evolving as a prognosti...
Quantification of tumor-specific variants (TSVs) in cell-free DNA is rapidly evolving as a prognosti...
Quantification of tumor-specific variants (TSVs) in cell-free DNA is rapidly evolving as a prognosti...
Quantification of tumor-specific variants (TSVs) in cell-free DNA is rapidly evolving as a prognosti...
Quantification of tumor-specific variants (TSVs) in cell-free DNA is rapidly evolving as a prognosti...
Next-generation sequencing (NGS) has rapidly replaced Sanger sequencing as the method of choice for ...
<p>Sanger sequence validation of highly expressed novel somatic SNVs for <i>MRPL3</i> variant in the...
<p>(A) Mapped reads of a Patient with RCMD loaded in the IGV browser (Version 2.3.34). <i>TET2</i> p...
Next-generation sequencing (NGS) has enabled the high-throughput discovery of germline and somatic m...
<p>FN: false negative result, extra: additional low allele frequency variants identified compared to...
The accurate detection of low-allelic variants is still challenging, particularly for the identifica...
Next-generation sequencing (NGS) has enabled the high-throughput discovery of germline and somatic m...