Retrograde signaling and nitrogen starvation regulators mediate the sporulation efficiency variation in <i>MKT1(89G)</i>-dependent manner.

<p>(A) Bar plots represent the mean sporulation efficiency, after 48h, of single gene deletions of <i>rtg1</i>∆, <i>rtg3</i>∆, <i>dal82</i>∆ both in M and S strains and double deletion of <i>rtg3∆ dal82∆</i> in M strain. wt indicates wild type strain. The sporulation efficiency data is indicated as circles. A pair and an interaction test using logit link function were performed (see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005195#sec008" target="_blank">Methods</a>). (B) Comparison of respiration for the yeast cells incubated for 1h in sporulation medium in the wild type M and S strains, and <i>rtg3∆</i> and <i>dal82∆</i> in the M strain. Y-axis denotes O₂ flux/vol (pmol/(s.ml)). <i>P</i> was calculated by an unpaired t-test. Error bars are the standard errors of mean.