ANKRD49 induces autophagy in serum-starved GC-1 cells.

<p>(A) The expression level of ANKRD49 in GC-1 stable cells. Left: mRNA levels, right: protein levels. (B) ANKRD49-induced autophagy, detected by the presence of Beclin 1, LC3-I to LC3-II conversion and p62 were analysed by Western blot; Rapamycin served as an autophagy-positive control and Wortmannin served as an autophagy-negative control, β-actin served as a loading control. One representative of three independent experiments is shown. The quantitative results are presented as the ratio of LC3-II to LC3-I (n = 3). **<i>p</i> < 0.01 indicates significant difference between groups as shown.