MCD diet-induced steatohepatitis is associated with increased miR-155 expression in parenchymal and non-parenchymal cells.

<p>C57Bl/6 mice were fed with methionine-choline deficient (MCD) or supplemented (MCS) control diet for 3, 6 and 8 weeks. Serum alanine aminotransferase levels (A) and TNFα mRNA expression (B) were determined (n = 5-8/group). Liver fibrosis was assessed by Sirius Red staining (100x; n = 3-6/group), representative slides are shown (C). miR-155 expression was detected by qPCR in total livers (n = 5-8/group) (D). Primary murine hepatocytes (n = 7/group), Kupffer cells (n = 6, pooled data, 2 datapoints/group) and liver mononuclear cells (LMNC; n = 3-4/group) were isolated from a subset of mice after 6 weeks of MCS or MCD diet feeding and cell-specific miR-155 expression was determined and represented as 1/dCt (E). miR-155* expression was determined in total livers (F). (*) indicates p<0.05 MCS vs. corresponding MCD group. Statistics was performed on fold change data.