Expression of selenoprotein and selenoprotein factor mRNAs activities and Sepsecs in liver of <i>Alb-Cre; Secp43</i>^<i>fl/fl</i> and control mice.

<p>(A) Selenoprotein mRNA levels were determined by qPCR. <i>18S</i> rRNA served as control. No significant differences were observed between genotypes (Student’s <i>t</i> test). Number of animals: male controls (n = 5), male mutants (n = 4), female controls (n = 4–6), female mutants (n = 6–7). (B) Selenoprotein biosynthesis factor mRNA levels were determined by qPCR. <i>18S</i> rRNA served as control. No significant differences were observed between genotypes (Student’s <i>t</i> test). Number of animals: male controls (n = 5), male mutants (n = 4), female controls (n = 4–6), female mutants (n = 6–7). (C) Sepsecs levels were determined by Western blotting. A protein of 48 kDa was detected in liver. No change was observed in the <i>Secp43</i> knockout.