Identification and characterization of simple sequence repeat (SSR) markers derived in silico from Brassica oleracea genome shotgun sequences
- Burgess, Benjamin
- Mountford, Hayley
- Hopkins, Clare J.
- Love, Cristopher
- Ling, Alison E.
- Spangenberg, German C.
- Edwards, David
- Batley, Jacqueline
Publication date
December 2006
DOI Publisher
Wiley ISSN
1471-8278 Citation count (estimate)
27Abstract
The availability of sequence data derived from shotgun sequencing programs enables mining for simple sequence repeats (SSRs), providing useful genetic markers for crop improvement. This study presents the development and characterization of 40 SSR markers from Brassica oleracea shotgun sequence and their cross-amplification across Brassica species. The markers show reliable amplification, genome specificity and considerable polymorphism, demonstrating the utility of SSRs for genetic analysis of commercial Brassica germplasm
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