Change in bidirectional pRNA transcription in HeLa after S1 or S5 dsRNA transfection.

<p>The levels of sense and antisense pRNAs were checked using directional qRT-PCR using P2 primer. Fold changes in sense and antisense pRNA expression were calculated using delta-delta-Ct method with respect to 18S as internal Control. Control: Control dsRNA treated cells, S1P2F: cells transfected with S1 dsRNA followed by RNA isolation and reverse transcription with P2F primer, S1P2R: cells transfected with S1 dsRNA and RNA isolated was reverse transcribed with P2R primer, S5P2F: cells transfected with S5 dsRNA and RNA isolated was reverse transcribed with P2F primer, S5P2R: cells transfected with S5 dsRNA and RNA isolated was reverse transcribed with P2R primer.