Effect of sense and antisense pRNA downregulation on dsRNA mediated TGS and TGA.

<p>HeLa cells were transfected with sense or antisense ODN and 24 hours later transfected with respective dsRNAs. RNA was isolated from the cells and analyzed by Real-Time PCR. Expression ratio was calculated with respect to 18S, POLR2A, PPIA and β-actin by REST software. (A) Hela cells transfected with antisense ODN. (B) HeLa cells transfected with sense ODN. Control-dsRNA: Control dsRNA transfected cells, S1-dsRNA: S1 dsRNA transfected cells, S5-dsRNA: S5 dsRNA transfected cells, S9-dsRNA: S9 dsRNA transfected cells.