Deletion of calponin-3 results in exencephaly.

<p>A. Targeted <i>Cnn3</i> locus before and after Cre-mediated deletion, illustrated as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0128385#pone.0128385.g002" target="_blank">Fig 2A</a>. B. Reflected light images of <i>Cnn3</i> ko d/d embryos their +/+ littermates as a control. C. Southern blot analysis of a control mouse (+/+), a heterozygous <i>Cnn3</i> (+/d) and a homozygous <i>Cnn3</i> knockout mouse (d/d). BamHI-digested genomic DNA from the respective mice was separated by gel electrophoresis, blotted and hybridized with the 3’ external probe. Arrows indicate the positions of fragments corresponding to the wild-type (+) as well as the deleted (d) allele. D. Fetal brain tissue of a homozygous <i>Cnn3</i> knockout and a control littermate was lysed, subjected to SDS-PAGE and western blotting and probed for calponin-3 and actin expression as a control.