<p>CSCs transduced homogenously with MyoT were cultured for 14 days ± Dox, then were analyzed by immunocytochemistry as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0125384#pone.0125384.g004" target="_blank">Fig 4</a>. A: Representative images. Bar, 20 μm. B: Prevalence of cardiac protein expression in the MYOCD-IRES-GFP<sup>nuc</sup>-positive, TBX5-IRES-dsRed<sup>nuc</sup>-positive cells. Three samples were analysed for each protein (≥ 1600 cells). Data are the mean ± SD of three independent experiments (14d) or are single experiments for 2 and 7d. p < 0.01 for all proteins at 14 versus 0 days. Bar, 20 μm.</p
Cardiac transcription factors are master regulators during heart development. Some were shown to tra...
<div><p>Cardiac transcription factors are master regulators during heart development. Some were show...
<p>(<b>A</b>) COS-7 cells were transfected with expression plasmids encoding Myc-tagged Tbx5 and a c...
<p>A: QRT-PCR comparing MEF2C alone (the GMT factor absent from the CSCs), the GMT triad, and the 4-...
<p>A: QRT-PCR showing stable expression of exogenous cardiac transcription factors in CSCs transduce...
<p>A: Timeline of the protocol, highlighting the use of rtTA-Puro<sup>r</sup> CSCs and secondary tra...
<p>QRT-PCR comparing <i>MYOCD</i> alone with <i>GATA4</i>, <i>MEF2C</i>, and <i>TBX5</i> in the abse...
<p><b>A–C</b>. GFP(+) cells were readily detected in MEFs transduced with either <i>G<sub>4</sub>T<s...
<p><b>A–I.</b> Induction of TF overexpression for 7 days in MEFs transduced with only FUW.M2rtTA or ...
<p>(<b>A</b>) COS-7 cells were transfected with expression plasmids encoding Flag-tagged Tbx5 and My...
<p>A: Validation of specificity was confirmed using mouse versus human TaqMan Gene Expression Assays...
<p>Here and throughout, Lin<sup>-</sup> / Sca1<sup>+</sup> / SP<sup>+</sup> CSCs were isolated from ...
<p>A: Timeline for the cardiogenic differentiation assays. CSCs were seeded in 6-well dishes (day 0)...
<p>10T1/2 fibroblasts were infected with Tbx5, Gata4, Myocd lentivirus alone, or different combinati...
<p>(A) Immunofluorescence staining of cTnT in the rBMSCs of the negative control group on the 14<sup...
Cardiac transcription factors are master regulators during heart development. Some were shown to tra...
<div><p>Cardiac transcription factors are master regulators during heart development. Some were show...
<p>(<b>A</b>) COS-7 cells were transfected with expression plasmids encoding Myc-tagged Tbx5 and a c...
<p>A: QRT-PCR comparing MEF2C alone (the GMT factor absent from the CSCs), the GMT triad, and the 4-...
<p>A: QRT-PCR showing stable expression of exogenous cardiac transcription factors in CSCs transduce...
<p>A: Timeline of the protocol, highlighting the use of rtTA-Puro<sup>r</sup> CSCs and secondary tra...
<p>QRT-PCR comparing <i>MYOCD</i> alone with <i>GATA4</i>, <i>MEF2C</i>, and <i>TBX5</i> in the abse...
<p><b>A–C</b>. GFP(+) cells were readily detected in MEFs transduced with either <i>G<sub>4</sub>T<s...
<p><b>A–I.</b> Induction of TF overexpression for 7 days in MEFs transduced with only FUW.M2rtTA or ...
<p>(<b>A</b>) COS-7 cells were transfected with expression plasmids encoding Flag-tagged Tbx5 and My...
<p>A: Validation of specificity was confirmed using mouse versus human TaqMan Gene Expression Assays...
<p>Here and throughout, Lin<sup>-</sup> / Sca1<sup>+</sup> / SP<sup>+</sup> CSCs were isolated from ...
<p>A: Timeline for the cardiogenic differentiation assays. CSCs were seeded in 6-well dishes (day 0)...
<p>10T1/2 fibroblasts were infected with Tbx5, Gata4, Myocd lentivirus alone, or different combinati...
<p>(A) Immunofluorescence staining of cTnT in the rBMSCs of the negative control group on the 14<sup...
Cardiac transcription factors are master regulators during heart development. Some were shown to tra...
<div><p>Cardiac transcription factors are master regulators during heart development. Some were show...
<p>(<b>A</b>) COS-7 cells were transfected with expression plasmids encoding Myc-tagged Tbx5 and a c...