Species-specific detection of ectopic cardiac transcription factors in CSCs.

<p>A: Validation of specificity was confirmed using mouse versus human TaqMan Gene Expression Assays to detect the indicated endogenous factors in the E14 embryonic mouse heart (blue) and 12-week human fetal heart (red). Data are the mean ± SD of three independent experiments. B: Human factor expression in adult mouse CSCs. Transduced cells were treated with Dox for 14 days. Upper low, results comparing transduction of MT, GMT, and MyoGMT; lower row, results comparing single transduction of the GMT factors versus co-transduction with <i>MYOCD</i>. Expression was typically decreased as the number of co-transducing viruses increased. *, p < 0.05 for the treated CSCs versus human fetal heart, for reference.