Suppression of calpain-1 corrected the imbalance of PPi metabolism in RVSMCs treated with oxLDL.

<p>RVSMCs were treated with or without oxLDL in the presence or absence of calpain-1 siRNA. The parameters were measured as described in Materials and Methods. A: PPi content in medium; B: ATP content in medium; C: ATP content in RVSMCs; D: ALP activity in medium; E: ALP activity in RVSMCs; F: ALP mRNA expression in RVSMCs; G: ALP protein expression in RVSMCs (upper: representative photograph; lower: statistical results); H: Enpp1 mRNA expression in RVSMCs; I: Ank mRNA expression RVSMCs. Results were expressed as mean±S.D. n = 4. *: <i>P</i><0.05 was considered statistically significant.