Cytokine requirements and timing of brain microvessel cross-presentation during PbA infection.

<p>(A–C) WT mice and mice deficient for IFNγ (A), TNFα (B), and LTα (C) were infected with PbA. After 7 days, brain microvessels were isolated from the infected mice as well as naïve WT mice, and incubated overnight with LR-BSL8.4a reporter cells. The total number of blue cells (counted as blue spots by the ELISOT reader) resulting from each brain after X-gal staining was quantified. *<i>P</i><0.05, ****<i>P</i><0.0001, ns not significant, ANOVA with Bonferroni’s post-test on log-transformed numbers. (D) Brain microvessels from uninfected mice and WT mice infected with PbA 5, 6, or 7 days previously were tested for cross-presentation as above. **<i>P</i><0.01, ****<i>P</i><0.0001, ns not significant as compared to uninfected mice by ANOVA with Bonferroni’s post-test on log-transformed numbers.