Interactions between CD8⁺ T cells and MBECs.

<p>(A) IFNγ-stimulated MBECs were incubated with (or without) 3 × 10⁶ thawed PbA mature iRBCs for 24 h. After washing, we added 10⁶ CD8⁺ T cells from either a naïve mouse or one infected with PbA 6 days previously. The wells were washed gently and photographed (DIC) after 20 h. Images are representative of triplicate wells. (B) An olfactory bulb smear from a mouse with ECM was fixed and stained with antibodies against von Willebrand factor (green) and CD8 (red). CD8⁺ T cells were present within blood vessels (arrows), on the abluminal face of endothelial cells (arrow head) and in the parenchyma (asterisks), 40× objective. Inset: a perivascular CD8⁺ T cell in close contact with the endothelium (100× objective).