Blebbistatin inhibited hypergravity-induced effects on the cytoskeleton and focal adhesions.

<p>(A) After treated with blebbistatin, non-cortical actin alignment disappeared (Scale bar = 10 μm). (B) Blebbistatin inhibited the hypergravity-induced increase in the mean fluorescence intensity of actin fibers. ~250 cells were counted (mean ± SEM). (C) Blebbistatin treatment reduced the numbers of actin fibers in cells. ~50 cells were counted (mean ± SEM). (D) Western blotting results of p-FAK/FAK after blebbistatin treatments. (E) Statistical analyses of results in D (n = 6). n.s. indicates no statistical difference between 1 ×<i>g</i> and 20 ×<i>g</i>. *, statistically significant difference (<i>P</i> < 0.05). Blebbistatin (Bleb).