Subcellular localization of grouper iridovirus caspase recruitment domain (GIV-CARD) proteins in HeLa cells.

<p>Recombinant GIV-CARD-EGFP proteins (green fluorescence) were detected at 6 h (A), 12 h (B) and 18 h (C). DAPI (blue fluorescence) was used to stain nuclei (D, E, and F). Panels G, H, and I are merged images of panels A and D, panels B and E, and panels C and F, respectively. GIV-CARD-FLAG recombinant proteins (red fluorescence) were detected by immunocytochemical staining and shown for the indicated times (J, K, and L). DAPI (blue fluorescence) was used to stain nuclei (M, N, and O). Panels P, Q, and R are merged images of panels J and M, panels K and N, and panels L and O, respectively. Scale bar = 10 μm.