Platelet PI3Kγ contributes to activated platelet-induced overexpression of proinflammatory mediators in the carotid artery wall after partial ligation.

<p>(A, B) Representative images of ICAM-1 and VCAM-1 immunofluorescence staining for left common carotid arteries from WT mice treated with PBS, WT platelets, or PI3Kγ^-/- platelets after partial ligation at 3 d, as well as their quantitative analysis (n = 5 per group). Scale bars: 50 μm. (C) mRNA levels of VCAM-1, ICAM-1, TNF-α, and IL-6 were determined by quantitative RT-PCR in left common carotid arteries from WT mice treated with PBS, WT platelets, or PI3Kγ^-/- platelets after partial ligation at 3 d (n = 5 per group). mRNA levels are normalized to GAPDH. * <i>P</i><0.05 versus vehicle group, # <i>P</i><0.05 versus WT platelet-infused mouse.