Platelet PI3Kγ mediates ADP-induced platelet activation through Akt- and p38 MAPK-dependent mechanisms.

<p>(A, B) Western blot analysis of phosphorylated p-p38 and p-Akt in platelets stimulated with ADP (20 μmol/L), with total p38 and Akt protein as internal controls. Average densitometric values normalized against those of internal control from three independent experiments are shown in the bar graph. Density from WT resting platelet sample was set as 1.0. Data are expressed as mean ± SEM. (C) Representative flow cytometric analyses of P-selectin, CD147, and CD154 expressions on WT platelet surface pretreated with Akti-1/2 (5 μmol/L) or SB203580 (10 μmol/L) for 5min, followed by stimulation with or without ADP (20 μmol/L) for 15 min, as well as the statistical data analysis from three separate experiments.