<i>In vivo</i> cellular immunogenicity of MVA deletion mutants, lacking fifteen genes, with 85A antigen.

<p>Four groups of female BALB/c mice (n = 4) were immunized (i.m.) with the respective MVAs at the dose of 2x10⁶pfu/ml. Seven days post immunization, intracellular cytokine staining and flow cytometry was performed to determine the percentage of splenic IFN-γ-secreting T cells in response to <i>in vitro</i> re-stimulation with MVA vector-specific peptide (<b>A</b> and <b>B</b>), with CD8⁺ T cell specific 85A peptide pool <b>(C)</b>, or with CD4⁺ T cell specific 85A peptide pool <b>(D)</b>. Two individual peptides were incubated for 18 hours to determine the CD8⁺<b>(E)</b> or CD4⁺ T <b>(F)</b> cell responses by ELISpot. These values are presented after subtracting the values of unstimulated cells for every mouse (sample). The mean of each group with the SEM error bars are shown. Data is representative of two independent experiments. There was no statistical significant difference in any of MVA mutant groups as compared to MVA85A or MVA-BAC85A groups, using Kruskal-Wallis test with Dunn's multiple comparisons. <b>MVA<i>wt</i>:</b> MVA wild type, <b>MVA85A:</b> MVA expressing TB 85A antigen, <b>MVA-BAC85A:</b> MVA, containing BAC DNA and expressing TB 85A antigen, and <b>∆15-MVA-85A:</b> MVA mutant, containing BAC DNA and expressing TB 85A antigen, with 15 gene deleted (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0128626#pone.0128626.t001" target="_blank">Table 1</a>).