ELISpot assay to assess subset of PBMCs stimulated by TpUB05 to produce interferon-gamma.

<p>CD4+ and CD8+ T-cells were separated from three (3) animals and stimulated with r-TpUB05 using bovine ELISpot assay. The CD4+ subpopulations of BH055 and BA219 appear to be significantly stimulated by TpUB05 (<i>p</i> = 0.013) compared to their CD8+ subpopulations. The CD4+ T-cells of BB007 did not significantly produce a higher amount of IFN-γ compared to its CD8+ T-cells subset. Comparing the response of all three animals tested, CD4+ T-cells produced a higher amount of IFN-γ (<i>p</i> = 0.046) compared to the CD8+ T-cells. An SI>2 is considered positive. The experiment was run in triplicates.