The <i>mms19</i> mutants cause an early flowering phenotype by affecting the expression of flowering-related genes.

<p>(A) The early flowering phenotype of <i>mms19</i> mutants in standard long-day conditions (16-h-light and 8-h-dark at 22°C). (B) The early-flowering phenotype was restored by the <i>MMS19-Myc</i> construction in <i>mms19-2</i>. (C) The statistics of total leaf numbers upon flowering under long-day conditions in the wild type, the <i>mms19</i> mutants and the complementation lines. T2-5 and T2-6 were two randomly selected individual <i>MMS19-MYC</i> transgenic lines in T2 generation. At least 30 individual plants were counted. Error bars stand for SD. Asterisks indicate significant difference as determined by the <i>t</i>-test (P<0.05). Numbers of rosette and cauline leaves are indicated by blue and red bars, respectively. (D) Leaf numbers under long-day conditions with or without vernalization. (E) Leaf numbers under short-day conditions. (F), (G) and (H) The effect of <i>mms19</i>, <i>abo4</i>, and <i>icu2</i> on the expression of flowering-related genes as determined by quantitative RT-PCR. <i>ACT7</i> was amplified as an internal control. The quantitative RT-PCR experiments were biologically repeated for three times and indicated similar results. A representative repetition is shown. Error bars represent SD. Asterisks show significant difference as determined by the <i>t</i>-test (P<0.05).