Chromatin Immunoprecipitation of histone modifications across Exon 1a and Exon 1b/c transcriptional start sites of PGC-1α.

<p>(A) Presence of an activating histone modification (H3K4me3) was examined via qPCR 1000bp in each direction from the transcriptional start site of Exon 1a (left) or Exon 1b/c (right). (B) Presence of a repressing histone modification (H3K27me3) was examined via qPCR 1000bp in each direction from the transcriptional start site of Exon 1a (left) or Exon 1b/c (right). 2-way ANOVA analysis found significant difference between the level of H3K4me3 only across the Exon 1b/c transcriptional start site (p<0.0001). Individual points were compared for statistical significance using student’s t-test (* p<0.05, ** p<0.005).