The induction of S100A7 expression in HCC94 cells in confluent culture.

<p>S100A7 expression was examined by immunohistochemistry (A, C, E), real-time PCR (G) and Western blotting (H). Cells were also examined by immunohistochemistry with nonspecific IgG (B, D, F).*, p<0.05, and **, p<0.01 versus the experimental groups and the pre-confluent groups. The results represent the average values from three independent experiments. N: pre-confluence; C6: cells were cultured at confluence for 6 days; C6-R3P: cells that were cultured at confluence for six days and then reseeded at sub-confluence for 3 cell passages.