Electron microscopy images show the subcellular distribution of immunoreactive vGluT2 in neural profile of a representative young VEH treated rat.

<p>Images were taken from the neuroprofile zone of the lateral median eminence, a region in which neurosecretory axons and GnRH dendrons are located. (A) Three puncta were framed and shown in higher magnification in panels B, C, and D. (B—D) The 10 nm immunogold labels for vGluT2 were seen on large dense-core vesicles (arrowheads). (E, F) Two representative adjacent sections from a series show consistent immunogold labeling of vGluT2 on large dense-core vesicles (white and black arrowheads). Glia = glial processes, Mit = mitochondria. Scale bar panel A = 2 μm, panel F (applies to B–F) = 200 nm.