Serological analyses.

<p>Antibody responses of i.p. infected mice, vaccinated pre- or post-parasite challenge, were measured by ELISA using affinity-purified Em2 (G11) and rEmP29 antigen. Bars indicate SE. Immunization and then infection: mouse groups from experiment no. 2 were immunized with different preparations (Saline solution (NaCl), saponin (Sap), rEmP29 plus saponin (rEmP29-Sap) or with rNcMIC1 plus saponin (rNcMIC1-Sap)), and then challenged by infection with an <i>E</i>. <i>multilocularis</i> metacestode suspension. Infection and then immunization: mice from experiment no. 3 were infected with <i>E</i>. <i>multilocularis</i> metacestode suspension, and at 1 month p.i., animals were treated with different preparations (physiological saline solution (NaCl), saponin (Sap), rEmP29 plus saponin (rEmP29-Sap) or with rNcMIC1 plus saponin (rNcMIC1-Sap)).