CCL2 alters the structure of the intestinal brush border and terminal web in living animals.

<p>(A-E) <i>C</i>. <i>elegans</i> L4 larvae of the indicated genotype were fed for 24 h on control (empty vector) or CCL2-expressing <i>E</i>. <i>coli</i>. Various fluorescent transgenic reporters were used to detect changes in intestinal cell architecture by confocal microscopy. (A) PGP-1::GFP is expressed at the intestinal apical plasma membrane. (B) RAB-8::GFP labels the intestinal cytoplasm. (C) ERM-1::GFP links the intestinal apical plasma membrane to the actin filaments within the microvilli. (D) ACT-5::GFP labels actin filaments within the microvilli and the terminal web underlying the intestinal apical plasma membrane. (E) IFB-2::CFP labels intermediate filaments in the terminal web. Scale bar: 10 μm; inset: 2x magnification of the lumenal section of the intestine.