Regulation of HIF-1α on ZEB1 and their expressions in CRC.

<p>(A) Endogenous expression levels of HIF-1α and ZEB1 in the indicated five cell lines were detected by qPCR with GAPDH used as the internal control. (B&C) HT29 cells were transduced with Ad5-HIF-1α or control viruses for 48 hours, following by qPCR and western blot to detect the expression of HIF-1α and ZEB1. GAPDH was used as the internal control. (D) HIF-1α and ZEB1 protein levels in matched nonneoplastic/cancerous colorectal tissues. N: normal; T: tumor. The level of each protein was normalized against GAPDH. (E) Representative pictures of IHC staining on human CRC formalin-fixed paraffin-embedded samples for HIF-1α and ZEB1. Left panel showed the adjacent normal colorectal tissues. Right panel showed CRC specimens. Original magnification, 200X.