Decreased Col3a1 mRNA expression after stopping Ang II infusion, increased Collagen III protein levels.

<p>Col3a1 mRNA (A) and Collagen III protein (B) expression was increased under Ang II infusion. Eight weeks after stopping infusion with Ang II, mRNA levels of Col3a1 decreased to control levels. Protein expression of Collagen III was unaffected after infusion with Ang II, however eight weeks after cessation of Ang II protein levels of Collagen III increased (B). Collagen III protein expression of control rats also significantly increased at sacrifice compared to their own biopsy levels (B). At time of sacrifice, former AngII-infused rats had significantly higher Collagen III protein levels when compared to control (B). Representative photomicrographs of Collagen III stained renal sections (C). Representative examples of immunoblot analysis of collagen III of two tissue samples in triplicate (D) (###p<0.001 vs. control at time of biopsy; **p<0.01, ***p<0.001 vs. Ang II at time of biopsy, $p<0.05 vs. control at time of sacrifice).