Decrease in Col1a1 mRNA expression after stopping Ang II infusion, ongoing deposition of Collagen I proteins.

<p>Ang II infusion increased Col1a1 mRNA (A) expression. After withdrawal of Ang II, Col1a1 mRNA levels returned to control values. Protein expression of Collagen I, as measured by dot blot analysis, was unaffected after infusion with Ang II. However, eight weeks after cessation of Ang II protein levels of Collagen I increased (B). Collagen I protein expression of control rats also significantly increased at sacrifice compared to their own biopsy levels (B). At time of sacrifice, former AngII-infused rats had significantly higher Collagen I protein levels when compared to control (B). Representative photomicrographs of Collagen I stained renal sections (C). Representative examples of immunoblot analysis of Collagen I of two tissue samples in triplicate (D). (#p<0.05, ###p<0.001 vs. control at time of biopsy; *p<0.05, ***p<0.001 vs. AngII at time of biopsy, $<0.05 vs. control at time of sacrifice).